Multiplex stimulated Raman histology: a route towards the application to the clinical routine
Stimulated Raman Histology (SRH) can be used for label-free histological tissue analysis. However current approaches are limited to just two simultaneous Raman peaks, restricting chemical specificity and suffering from limited applicability to just fresh/fresh-frozen samples. Here we present a new approach to SRH, based on multiplex SRS technology, which is enabling deep FFPE-tissue biopsy analysis. The simultaneous detection of 38 channels across the entire CH spectrum (2800-3100 cm-1) in parallel allows chemometric and multimodal tissue analysis on all kind of tissue samples. By incorporating AI-driven virtual H&E staining and tissue segmentation for diagnostic purposes, it advances SRH towards clinical-diagnostic.
Authors
- Francesco Crisafi (ORCID: https://orcid.org/0000-0002-6577-7967)
- Moe Vali
- Anna Muehlig
- David Pertzborn (ORCID: https://orcid.org/0000-0002-0230-7765)
- Giulio Cerullo (ORCID: https://orcid.org/0000-0002-9534-2702)
- K. K. Mujeeb Rahman
- Píetro Lió (ORCID: https://orcid.org/0000-0002-0540-5053)
- M. Negro (ORCID: https://orcid.org/0000-0002-7317-6598)
- Daniela Pelzel
- Orlando Guntinas‐Lichius (ORCID: https://orcid.org/0000-0001-9671-0784)
- Renzo Vanna (ORCID: https://orcid.org/0000-0001-6218-8393)
- Federico Monti (ORCID: https://orcid.org/0000-0003-3736-891X)
- Andrea Ragni (ORCID: https://orcid.org/0000-0003-4227-570X)
- Gabriele Di Noia
- Tiago Azevedo
- Eleonora Erriquez
- Ulrike Weyer
- Anna Xylander
Institutions
- University of Cambridge (GB)
- Rama University (IN)
- Istituto Ramazzini (IT)
- Istituto di Fotonica e Nanotecnologie (IT)
- Jena University Hospital (DE)
- Politecnico di Milano (IT)
Publication Details
- Published
- 2025-03-19
- DOI
- https://doi.org/10.1117/12.3043003
- Primary Topic
- Spectroscopy Techniques in Biomedical and Chemical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00