Label-free nanoscale imaging in live cells with advanced confocal interferometric scattering microscopy (C-iSCAT)

We present an enhanced confocal interferometric scattering microscopy (iSCAT) technique for label-free imaging of live cells. By integrating confocal illumination and detection, iSCAT overcomes challenges related to speckle from complex scattering media, achieving high-quality nanoscale imaging. A unique scanning mirror system further improves field homogeneity and allows for higher acquisition rates. This method provides shot-noise limited detection and visualization of intracellular organelles without the need of a label. Our results demonstrate the potential of confocal iSCAT for long-term live cell studies, with the ability to combine with fluorescence microscopy when additional specificity is required.