Multicontrast deep ultraviolet transmission and autofluorescence camera microscopy

We demonstrate a camera-based deep ultraviolet (DUV) transmission and autofluorescence microscopy system which employs a 265/10 nm LED to generate high resolution virtual histology images of human prostate and breast FFPE samples. By detecting the transmitted deep ultraviolet radiation, pseudo-absorption images are generated which yield detailed nucleic contrast. Additionally, the emitted autofluorescence is simultaneously collected in various spectral bands to establish contrasts of endogenous fluorophores including collagen/elastin, NADH, and FAD. We envision that this combined DUV transmission and autofluorescence camera system could provide diagnostic utility in clinical and/or research settings.