Label-free refractive index mapping of human sperm cells
This study presents a method for refractive-index mapping of freely swimming human sperm cells using rapid tomographic phase microscopy combined with fluorescence imaging. By capturing tomographic data with shearing interferometry and a fast camera, we offer a detailed label-free visualization of sperm morphology and movement, enhancing sperm selection process in intracytoplasmic sperm injection (ICSI).
Authors
- Dotan Kambar
- Natan T. Shaked (ORCID: https://orcid.org/0000-0003-4080-839X)
- Simcha K. Mirsky (ORCID: https://orcid.org/0000-0003-3244-8742)
- Itay Barnea (ORCID: https://orcid.org/0000-0002-8590-8995)
- Ofira Dabah
- Lydia Sokolovski
Institutions
- Tel Aviv University (IL)
Publication Details
- Published
- 2025-03-19
- DOI
- https://doi.org/10.1117/12.3041226
- Primary Topic
- Optical Polarization and Ellipsometry
- Type
- article
- Field-Weighted Citation Impact
- 0.00