Light-sheet fluorescence microscopy and beating pattern detection for human heart organoid analysis
We demonstrate a custom-made light-sheet microscope based on tunable structured illumination (SI) frequencies using a grating light valve (GLV) for in-vivo cardiac organoid imaging. Furthermore, we also introduce three image processing techniques to characterize the beating patterns of sequential beating cardiac organoid images acquired by our custom-made light-sheet microscope.
Authors
- Yifan Liu (ORCID: https://orcid.org/0000-0003-2887-7704)
- Brett Volmert (ORCID: https://orcid.org/0000-0002-5209-4833)
- Aitor Aguirre (ORCID: https://orcid.org/0000-0002-1383-2792)
- Yonatan R. Lewis‐Israeli (ORCID: https://orcid.org/0000-0001-9993-0707)
- Cheng‐You Yao (ORCID: https://orcid.org/0000-0002-6045-8676)
- Aniwat Juhong (ORCID: https://orcid.org/0000-0002-9115-9767)
- Wibool Piyawattanametha (ORCID: https://orcid.org/0000-0002-2228-8485)
- Wen Li (ORCID: https://orcid.org/0000-0002-3851-7406)
- Bo Li (ORCID: https://orcid.org/0000-0002-2820-9098)
- Zhen Qiu (ORCID: https://orcid.org/0000-0001-8790-8481)
Institutions
- Michigan State University (US)
Publication Details
- Published
- 2025-03-19
- DOI
- https://doi.org/10.1117/12.3042166
- Primary Topic
- Cardiovascular Effects of Exercise
- Type
- article
- Field-Weighted Citation Impact
- 0.00