Single-cell imaging, single-spot optoretinography sensing (SCISSORS) in living human subjects

Optoretinography (ORG) is an emerging technique that records optical path length (OPL) changes in retinal cells at the nanometer scale in response to light stimuli. However, real-time scanning of the same patch of the retina is extremely challenging due to incessant eye movement, even during fixation. Here, we overcome eye movement limitations and demonstrate potential for single-cell imaging and single-spot optoretinography sensing (SCISSORS) in living human subjects. Using real-time eye motion correction via adaptive optics scanning light ophthalmoscopy (AOSLO), we were able to acquire ORGs in different scan modes with a level of flexibility and spatial control previously unattainable over an entire 5-second imaging session. The presented single-cell optoretinography, or SCISSORS, potentially opens new doors for vision science and ophthalmology.