Coupling a rebuild shuttle system with biosynthetic pathway and transcription factor engineering for enhanced <scp>l</scp>-cysteine production
A novel plasmid-free engineered E. coli strain was developed to significantly increase l -cysteine yield, achieving a titer of 35.54 g L −1 in a 5 L bioreactor.
Authors
- Zhi‐Qiang Liu (ORCID: https://orcid.org/0000-0003-3259-6796)
- Xue Cai (ORCID: https://orcid.org/0000-0001-5919-9817)
- Yu‐Guo Zheng (ORCID: https://orcid.org/0000-0002-6358-6243)
- Xin Sui (ORCID: https://orcid.org/0000-0002-6830-7012)
- Bo Zhang (ORCID: https://orcid.org/0000-0002-0350-244X)
- Lifeng Chen (ORCID: https://orcid.org/0000-0001-8421-269X)
- Yuhe Guo
- Xiao-Ling Xiu
- Ruyi Zheng (ORCID: https://orcid.org/0000-0002-7415-3160)
Institutions
- Zhejiang University of Technology (CN)
Publication Details
- Journal
- Green Chemistry
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5gc00433k
- Citations
- 2
- Primary Topic
- Metalloenzymes and iron-sulfur proteins
- Type
- article
- Field-Weighted Citation Impact
- 0.73
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Zhejiang Province