Constant Enzymatic <i>in situ</i> Production of H<sub>2</sub>O<sub>2</sub> for an Unspecific Peroxygenase by an <scp>l–</scp>Amino Acid Oxidase
Abstract Unspecific Peroxygenases have been the subject of a lot of research in the recent past as they offer opportunities for green hydroxylation reactions using hydrogen peroxide (H 2 O 2 ) as donor. A striking problem of UPOs is their low stability against their own co‐substrate H 2 O 2 . In order to prevent the deactivation, we developed an enzyme cascade with an l ‐amino acid oxidase (LAAO) to produce hydrogen peroxide in situ at a constant low concentration, aiming for much higher lifespan and therefore longer working time of the enzyme. This way, we are able to convert a variety of substrates with conversion rates up to 95%.
Authors
- Tobias Heinks (ORCID: https://orcid.org/0000-0003-3131-2106)
- Simon Koopmeiners (ORCID: https://orcid.org/0009-0007-0947-6231)
- Jan von Langermann (ORCID: https://orcid.org/0000-0001-9302-9803)
- Martin J. Weissenborn (ORCID: https://orcid.org/0000-0002-1200-4485)
- Gabriele Fischer von Mollard (ORCID: https://orcid.org/0000-0003-3236-1401)
- Simon Last (ORCID: https://orcid.org/0009-0005-8862-4209)
- N.L. Dietz
Institutions
- Bielefeld University (DE)
- Hochschule Bielefeld (DE)
- Martin Luther University Halle-Wittenberg (DE)
- Otto-von-Guericke-Universität Magdeburg (DE)
- Delft University of Technology (NL)
Publication Details
- Journal
- Advanced Synthesis & Catalysis
- Published
- 2025-03-17
- DOI
- https://doi.org/10.1002/adsc.202500105
- Citations
- 5
- Primary Topic
- Nitric Oxide and Endothelin Effects
- Type
- article
- Field-Weighted Citation Impact
- 5.82