Volatile Compositional Profile, Antioxidant Properties, and Molecular Docking of Ethanolic Extracts from Philodendron heleniae

Antioxidants are essential compounds with diverse applications, and medicinal plants are a natural source of these biomolecules. <i>Philodendron heleniae</i>, a species native to the Ecuadorian Amazon, belongs to a genus renowned for its traditional therapeutic uses. Extracts from the stems and roots of several <i>Philodendron</i> species have been widely used to treat stress, bladder disorders, and snakebite wounds, underscoring their medicinal potential. This study investigates the volatile composition, antioxidant properties, and molecular docking of ethanolic extracts from <i>P. heleniae</i>, aiming to expand its applications. Phytochemical analysis revealed a rich profile of tannins, phenolic compounds, flavonoids, and terpenoids. Antioxidant assays (ABTS and DPPH) demonstrated the extract's strong free radical scavenging capacity, comparable to the standard Trolox. GC-MS analysis identified 48 volatile and semi-volatile metabolites, predominantly phenolic compounds, terpenoids, and lipid-like molecules. Fractionation of the crude ethanolic extract into aqueous and ethanolic fractions simplifies the downstream analytical steps and facilitates the identification and the evaluation of the higher abundance of antioxidant-related metabolites. Molecular docking supported these findings, highlighting strong binding affinities of stigmasterol and desmosterol to catalase, an enzyme critical for reducing oxidative stress. These results position <i>P. heleniae</i> as a promising source of natural antioxidants with potential pharmaceutical applications, while emphasizing the importance of conserving Ecuador's biodiversity and its bioactive resources.