Development of an efficient expression system for human chaperone BiP in Pichia pastoris: production optimization and functional validation
A scalable bioprocess to produce rhBiP in P. pastoris was developed, providing a high yield of biologically active protein in a chemically defined mineral medium. It opens a source of rhBiP to accelerate further therapeutic applications of this important protein.
Authors
- Eimantas Žitkus
- Rimantas Slibinskas
- Andrius Sakalauskas (ORCID: https://orcid.org/0000-0002-2715-067X)
- Mantas Žiaunys (ORCID: https://orcid.org/0000-0002-8368-6188)
- Evaldas Čiplys (ORCID: https://orcid.org/0000-0003-0468-2451)
Institutions
- Vilnius University (LT)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-03-18
- DOI
- https://doi.org/10.1186/s12934-025-02679-z
- Citations
- 2
- Primary Topic
- Heat shock proteins research
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- European Regional Development Fund