Detection of CpG methylation based on the change in amplification efficiency of strand-displacement DNA polymerase by CpG methylation
The amplication efficiency of loop-mediated isothermal amplification (LAMP), which uses strand displacement DNA synthesis, was reduced by CpG methylation. This finding may contribute to the rapid and easy detection of CpG methylation.
Authors
- Kaori Tsukakoshi (ORCID: https://orcid.org/0000-0003-4779-1113)
- Kazunori Ikebukuro (ORCID: https://orcid.org/0000-0003-2838-0562)
- Mizuki Tomizawa
- Kiichi Watanabe (ORCID: https://orcid.org/0000-0001-6505-8535)
Institutions
- Tokyo University of Agriculture and Technology (JP)
Publication Details
- Journal
- Sensors & Diagnostics
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5sd00012b
- Primary Topic
- Epigenetics and DNA Methylation
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Tokyo University of Agriculture
- Tokyo University of Agriculture and Technology