Introducing Rigidity into the GFP Chromophore via a Boron Bridge: Insights and Application in Two-Photon Imaging
A new family of azaborine fluorophores, consisting of ten compounds, has been developed that mimics the chromophore of green fluorescent protein, with conformation locked by a hydroxyboron bridge. These fluorophores exhibit fluorescence Stokes shifts up to 100 nm (0.56 eV) and high brightness (>10<sup>4</sup> M<sup>-1</sup> cm<sup>-1</sup>) in the 408-525 nm range. Their potential utility for sensing applications was demonstrated by constructing zinc sensors capable of detecting 100 micromolar Zn<sup>2+</sup> concentrations in biological systems with two-photon microscopy.
Authors
- Balázs Rózsa (ORCID: https://orcid.org/0000-0003-1427-7003)
- Ervin Kovács (ORCID: https://orcid.org/0000-0002-3939-6925)
- Attila Csomos (ORCID: https://orcid.org/0000-0002-4955-5002)
- Arnold Steckel (ORCID: https://orcid.org/0000-0002-4423-0399)
- Gábor Turczel (ORCID: https://orcid.org/0000-0002-6753-6796)
- Zoltán Mucsi (ORCID: https://orcid.org/0000-0003-3224-8847)
- Gitta Schlosser (ORCID: https://orcid.org/0000-0002-7637-7133)
- Levente Cseri (ORCID: https://orcid.org/0000-0002-3016-5477)
- Anett Matuscsák
- Brigitta Petrilla
Institutions
- Eötvös Loránd University (HU)
- University of Miskolc (HU)
- Pázmány Péter Catholic University (HU)
- Brainvest (Hungary) (HU)
- Institute of Materials and Environmental Chemistry (HU)
- HUN-REN Institute of Experimental Medicine (HU)
- University Of Information Technology (MM)
- Czech Academy of Sciences, Institute of Experimental Medicine (CZ)
- Centre for Structural Systems Biology (DE)
- HUN-REN Research Centre for Natural Sciences (HU)
Publication Details
- Journal
- Organic Letters
- Published
- 2025-03-18
- DOI
- https://doi.org/10.1021/acs.orglett.5c00284
- Citations
- 1
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 1.63
Funders
- Magyar Tudományos Akadémia
- Nemzeti Kutatási Fejlesztési és Innovációs Hivatal
- H2020 Leadership in Enabling and Industrial Technologies