<i>Aloe Schweinfurthii</i> Gel in the Safe Preservation of Living Periodontal Cells

Introduction: To address the public health challenges related to the preservation of expelled immature permanent teeth, we have pushed our research towards alternative plant-based media. Plants have always been vital to humanity. This is the case of Aloe which is known for its great diversity with different biological activities. The species chosen for our study is Aloe schweinfurthii. The purpose of the survey was to conduct research on cost-to-dose, accessibility, efficacy and safety. Our overall goal was to evaluate the toxicity of Aloe schweinfurthii gel in order to safely ensure the survival of periodontal ligament cells from an expelled immature permanent tooth. Methods: This experimental study involved acute and subacute toxicity. We evaluated its effects on the weight parameters of the animals, liver, kidney and hematological as well as the histopathology of the organs. Statistical analyses were done by SPSS version 23.0. The tests performed were ANOVA, Student and Newman-Keuls. The values of p <0.05 were considered statistically significant in all tests. The figures were made by Graph pad prism version 8.0.1. Results: The administered A. schweinfurthii did not cause any animal death, nor did they show any obvious sign of toxicity. Weight gain in rats in acute and subacute toxicity was observed. A decrease in liver, lung and brain weight was noted. We noted also an increase in the rate of ALAT, ASAT, serum creatinine, serum uric acid, white blood cells at satellite doses. Histopathology of the organs showed no cellular disorganization, nor damage to the hepatic and renal tissues. Preservation of cellular vitality was observed at all minimum concentrations from 24 to 72 hours in complete safety. Conclusion: Acute and subacute toxicity tests were unremarkable. A. schweinfurthii gel appears to be a good medium for the safe preservation of periodontal ligament cells.