Using short-read 16S rRNA sequencing of multiple variable regions to generate high-quality results to a species level
This protocol provides an effective means for studying infant gut microbial samples at a species level. However, sample collection approaches need to be accounted for in any downstream analysis.
Authors
- Amy S. Graham (ORCID: https://orcid.org/0000-0002-5956-8341)
- M. Holmes (ORCID: https://orcid.org/0000-0001-5873-2405)
- Fadheela Patel
- Mamadou Kaba
- Francesca Little (ORCID: https://orcid.org/0000-0002-5917-9928)
- André van der Kouwe (ORCID: https://orcid.org/0000-0002-2754-6594)
Institutions
- Harvard University (US)
- University of Cape Town (ZA)
- Simon Fraser University (CA)
- Massachusetts General Hospital (US)
- Athinoula A. Martinos Center for Biomedical Imaging (US)
Publication Details
- Journal
- Frontiers in Bioinformatics
- Published
- 2025-03-17
- DOI
- https://doi.org/10.3389/fbinf.2025.1484113
- Citations
- 1
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Institutes of Health