Effect of Polyhemoglobin and Synthetic Enzymes on Ischemic Cardiomyocytes

We studied the effect of a novel solution consisting of polyhemoglobin together with three synthetic enzymes (neocatalase, neosuperoxide dismutase, and neocarbonic anhydrase) on ischemic human cardiomyocytes. Polyhemoglobin alone showed minimal protective effects. The addition of the three synthetic enzymes to polyhemoglobin provided antioxidant protection, reduced Reactive Oxygen Species (ROS) levels, and improved cellular recovery. ROS production and mitochondrial function assays confirmed significant reductions in oxidative damage with the combined treatment. Apoptosis and necrosis detection showed improved cell survival, while cardiac troponin assays indicated reduced myocardial injury. The novel solution of polyhemoglobin with all three synthetic enzymes provided the best protection compared to polyhemoglobin alone or polyhemoglobin combined with only two neoenzymes (neoCatalase and neoSuperoxide Dismutase). This study highlights the potential of polyhemoglobin with the three synthetic enzymes for the next generation blood substitutes and organ preservation as it offers a novel approach to improving the outcomes of ischemic cardiomyocytes.