Synthesis of PEG-Cobalt nanoparticles and study of the Antibacterial activity by using alternating magnetic field on <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>

Abstract After being created using the Sol-Gel method, the polyethylene glycol-Cobalt (PEG-Co) nanoparticles’ antibacterial activity was evaluated against Escherichia coli and Staphylococcus aureus . The functionalized nanoparticles were assessed by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), and a vibrating sample magnetometer (VSM). The PEG-Co was typically between 11 and 23 nm in size. Superparamagnetism and powerful saturation magnetization were also visible in the PEG-Co, at standard temperature. E. coli and S. aureus were tested for PEG-Co antibacterial activity using the agar well-diffusion method and the Release method. The findings demonstrate the effectiveness of PEG-functionalized magnetic (PEG-Co) nanoparticles with an alternating magnetic field (AMF) as a novel DNA-mediated antibacterial agent. Nucleic acid damage, loss of cell-wall integrity, and increased (cell-wall) permeability due to bacterial cytoplasmic membrane and nucleic acid breaching were found to be the mechanisms by which the (PEG-Co)+AMF nanoparticles killed the bacteria by the increase in temperature. Future biomedical and pharmacological uses of the (PEG-Co)+AMF nanoparticles may be used as a possible antibacterial agent.