Transcriptome analysis and molecular docking reveal the activation of FOXO4, TNFSF15 and CASP9 in HeLa cells treated with DCM fraction from <i>Clinacanthus nutans</i> ( <i>C. nutans</i> )

<i>Clinacanthus nutans</i> (<i>C. nutans</i>) is a well-known herb in tropical Asia. Previous studies have reported the anticancer activity of <i>C. nutans</i> but the molecular mechanisms on cervical cancer are not fully understood. Therefore, our study aims to explore its effects on HeLa line and analyse the molecular interactions. Firstly, the RNA-Seq reads were processed for differentially expressed analyses. With Ingenuity Pathway Analysis (IPA) method, five cell death-related pathways with three significant genes (CASP9, FOXO4, TNFSF15) were identified. This study provides insight into the potential role of the DCM fraction of <i>C. nutans</i> mediating cell death mechanism in cervical cancer.