Formation of Complex Spatial Structures of DNA in the Process of Ab Initio Synthesis

Products with a complex spatial organization were formed during ab initio synthesis using the large fragment of the Bst DNA polymerase. Using the analysis of AFM images of products obtained with the addition of a minimal amount of the nicking endonuclease Nt. BspD6I during this synthesis it was possible to describe the structures that formed as a result of this synthesis and suggest that their formation occurs by the mechanism of recombination-dependent replication.