Multi-platform omics analysis of Nipah virus infection reveals viral glycoprotein modulation of mitochondria
The recent global pandemic illustrates the importance of understanding the host cellular infection processes of emerging zoonotic viruses. Nipah virus (NiV) is a deadly zoonotic biosafety level 4 encephalitic and respiratory paramyxovirus. Our knowledge of the molecular cell biology of NiV infection is extremely limited. This study identified changes in cellular components during NiV infection of human cells using a multi-platform, high-throughput transcriptomics, proteomics, lipidomics, and metabolomics approach. Remarkably, validation via multi-disciplinary approaches implicated viral glycoproteins in enriching mitochondria-associated proteins despite an overall decrease in protein translation. Our approach also allowed the mapping of significant fluctuations in the metabolism of glucose, lipids, and several amino acids, suggesting periodic changes in glycolysis and a transition to fatty acid oxidation and glutamine anaplerosis to support mitochondrial ATP synthesis. Notably, these analyses provide an atlas of cellular changes during NiV infections, which is helpful in designing therapeutics against the rapidly growing Henipavirus genus and related viral infections.
Authors
- Brooke L. Deatherage Kaiser (ORCID: https://orcid.org/0000-0001-7039-1332)
- Lei Wu (ORCID: https://orcid.org/0000-0001-6553-6322)
- Kelly G. Stratton (ORCID: https://orcid.org/0000-0002-1721-9688)
- Cameron Casey (ORCID: https://orcid.org/0000-0001-6790-2170)
- Fikret Aydemir
- Viraj Upadhye (ORCID: https://orcid.org/0000-0002-6755-0029)
- Kristie Oxford (ORCID: https://orcid.org/0000-0002-8125-1391)
- Jason McDermott (ORCID: https://orcid.org/0000-0003-2961-2572)
- Jennifer Kyle (ORCID: https://orcid.org/0000-0002-9282-9005)
- Karl Weitz (ORCID: https://orcid.org/0000-0003-3453-1283)
- Heather Olson (ORCID: https://orcid.org/0000-0002-4501-9587)
- J. Lizbeth Reyes Zamora (ORCID: https://orcid.org/0000-0003-3000-817X)
- David W. Buchholz (ORCID: https://orcid.org/0000-0001-8874-6648)
- Heinz Feldmann (ORCID: https://orcid.org/0000-0001-9448-8227)
- Katrina M. Waters (ORCID: https://orcid.org/0000-0003-4696-5396)
- Joeva J. Barrow
- I. Abrrey Monreal (ORCID: https://orcid.org/0000-0003-0521-9063)
- Ruth Collins (ORCID: https://orcid.org/0000-0002-7513-2038)
- Gunner P. Johnston
- Emmie de Wit (ORCID: https://orcid.org/0000-0002-9763-7758)
- Hector C. Aguilar (ORCID: https://orcid.org/0000-0001-6879-8360)
- Joshua Adkins (ORCID: https://orcid.org/0000-0003-0399-0700)
- Natalie C. Heller
- Haewon Byun
- D H Goodall
Institutions
- National Institutes of Health (US)
- Pacific Northwest National Laboratory (US)
- Cornell University (US)
- Environmental Molecular Sciences Laboratory (US)
- National Institute of Allergy and Infectious Diseases (US)
- New York State University College of Human Ecology (US)
Publication Details
- Journal
- Cell Reports
- Published
- 2025-03-01
- DOI
- https://doi.org/10.1016/j.celrep.2025.115411
- Citations
- 1
- Primary Topic
- Virology and Viral Diseases
- Type
- article
- Field-Weighted Citation Impact
- 1.35
Funders
- U.S. Department of Energy
- U.S. Department of Homeland Security
- Battelle
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases
- National Institutes of Health
- Science and Technology Directorate
- National Institute of Allergy and Infectious Diseases
- Pacific Northwest National Laboratory