FORMULATION, CHARACTERIZATION AND EVALUATION OF DAIDZEIN-LOADED SOLID-LIPID NANOPARTICLES GEL FOR ANTI-INFLAMMATORY POTENTIAL

Objective: Daidzein is a natural isoflavone present in various leguminous plants. It has broad therapeutic effects, including cardioprotective, anticancer, antidiabetic, anti-inflammatory, and anti-oxidative effects. Objective of present study was to enhance the solubility and bioavailability of daidzein through the development of Solid-Lipid Nanoparticle (SLN) gel formulation for anti-inflammatory effect. Methods: Daidzein-loaded SLNs were prepared using ultrasonication solvent emulsification method and optimized SLNs were converted into gel using Carbopol 934. Prepared Daidzein Loaded Solid Lipid Nanoparticles (DAID–SLNs) gel was characterized for drug release, pH, viscosity and spreadability. DAID–SLNs gel was further evaluated for in vitro anti-inflammatory effect using albumin denaturation inhibition, anti-proteinase assay and anti-lipoxygenase assay. Results: Optimized SLNs were successfully converted into a homogeneous, semisolid gel with good spreadability. The percentage drug release was 61.2±3.01 in 180 min, while nanoparticle formulation DAID-SLNs showed 70.9±3.18 percentage release in 180 min. Optimized formulation showed small particle size (193.62±5.89 nm), polydispersity Index (0.21±0.42) and increased Zeta potential with negatively charged (-33.17±1.24 mV), which showed good physical stability of the formulation. In vitro anti-inflammatory effect in protein denaturation inhibition assay of DAID–SLNs loaded gel showed with 91.42±3.18% was comparable to Standard Diclofenac sodium showed 95.24±3.85%. Proteinase inhibitory effect at concentration of 100µg/ml was showed 92.46±3.45% that was comparable to the standard Diclofenac sodium (98.28±3.72 %). Conclusion: Daidzein-loaded solid lipid nanoparticles gel was found effective for treatment of inflammation through inhibition of protein denaturation and essential enzymes proteinase as well as lipoxygenase.