Benchtop Proton NMR Spectroscopy for High-Throughput Lipoprotein Quantification in Human Serum and Plasma
We report the successful development and translation of high-field nuclear magnetic resonance (NMR) based comprehensive lipoprotein analysis to routine benchtop systems. This demonstrates the potential to reimagine population level cardiovascular disease risk analysis and individual level screening based on blood sampling. Using a quantitative calibration approach, we obtained stable and reproducible results from multiple sites, despite reduced spectral dispersion and sensitivity at lower field strengths. Our study shows that 25 out of 28 major lipoprotein parameters, including key cardiometabolic risk markers, were faithfully measured using benchtop NMR systems within 15 min. This development has significant implications for making a powerful diagnostic tool widely available, enhancing the potential for longitudinal personalized medicine through molecular phenotyping in the clinic.
Authors
- Tammo Diercks (ORCID: https://orcid.org/0000-0002-5200-0905)
- Manfred Spraul
- Birk Schütz
- Jeremy K. Nicholson (ORCID: https://orcid.org/0000-0002-8123-8349)
- Ricardo Conde (ORCID: https://orcid.org/0000-0003-4372-081X)
- Maider Bizkarguenaga (ORCID: https://orcid.org/0000-0003-0988-2006)
- Philipp Nitschke (ORCID: https://orcid.org/0000-0002-5814-7529)
- Julien Wist (ORCID: https://orcid.org/0000-0002-3416-2572)
- Elaine Holmes (ORCID: https://orcid.org/0000-0002-0556-8389)
- Drew A. Hall (ORCID: https://orcid.org/0000-0003-0674-074X)
- Miriam Cobo (ORCID: https://orcid.org/0000-0001-9208-9062)
- Claire Cannet
- Óscar Millet (ORCID: https://orcid.org/0000-0001-8748-4105)
- Nieves Embade (ORCID: https://orcid.org/0000-0001-9878-3290)
- Wenjun Wang (ORCID: https://orcid.org/0000-0001-8898-0389)
- Samantha Lodge (ORCID: https://orcid.org/0000-0001-9193-0462)
- Zhonglin Chai (ORCID: https://orcid.org/0000-0001-8182-8579)
- Ángela de Diego
- Hartmut Schäfer
- Sudhir Kowlessur
- Niels Pompe
Institutions
- Murdoch University (AU)
- Diabetes Australia (AU)
- Australian Regenerative Medicine Institute (AU)
- Division of Chemistry (US)
- CIC bioGUNE (ES)
- Bruker (Germany) (DE)
- Ministry of Health and Quality of Life (MU)
- Imperial College London (GB)
- Monash University (AU)
- Universidad del Valle (CO)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2025-03-17
- DOI
- https://doi.org/10.1021/acs.analchem.4c04660
- Citations
- 11
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 8.87
Funders
- McCusker Charitable Foundation
- Spinnaker Health Research Foundation
- Australian Research Council
- National Health and Medical Research Council
- Agencia Estatal de Investigación
- Department of Jobs, Tourism, Science and Innovation, Government of Western Australia