Biochar Amendment Increases Peanut Production Through Improvement of the Extracellular Enzyme Activities and Microbial Community Composition in Replanted Field

Peanut yield and quality are often threatened by soil degradation under continuous cropping. Biochar has been known to improve the soil microbial community and plant resistance. However, studies on its functions to reduce soil degradation losses and improve the peanut yield are limited. A field peanut experiment was conducted in an Alfisol soil and biochar was applied at a rate of 20 t ha^-1 in 2022. The biochar was prepared from woodchip (WB) and maize straw (MB) feedstocks alone, as well as with co-composted biochar of the same feedstocks with pig manure labeled as WBSC and MBSC amendment, respectively. The conventional organic manure was applied as a control treatment (OM). All plots were base-fertilized with a mineral compound fertilizer of N-P₂O₅-K₂O (16-16-16, %) at 600 kg ha^-1. Topsoil (20 cm) and plant samples were collected at the time of peanut harvest. Soil quality, enzyme function, peanut growth traits, microbial abundance, and community composition were analyzed. Compared to OM, peanut yields increased by 22%, 23%, and 18% under WB, WBSC, and MBSC, respectively. The content of oleic acid increased by 4-5%, while the content of linoleic acid decreased by 7-9%, respectively, under biochar-compost treatments. However, biochar amendment alone showed non-significant changes in these fatty acids. The soil extracellular enzyme activity increased by 3.7-5.5% with biochar amendments and 6.4-10.1% with biochar-compost application. The enzyme activity ratio of hydrolase to non-hydrolase, of C cycling to N cycling, and of P cycling increased by 11.4-15.9%, 20.9-33.8%, and 14.7-23.5% under biochar amendments and by 20.5-25.0%, 17.4-39.0%, and 23.5-32.3% under biochar-compost, respectively. Overall, crop residue biochar enhanced peanut yield and quality by improving soil aggregation, enzyme functionality, and fungal community in line with the soil nutrient supply.