Control Levels of Tranlocations in Human Peripheral Blood Lymphocyte Cultures Determined Using Three-Color FISH Staining

This paper presents the results of using three-color FISH staining of chromosomes (DNA probes for chromosome pairs 1, 4, and 12) to study the control level of translocations in peripheral blood lymphocyte cultures of 35 relatively healthy volunteer donors aged from 21 to 72 years. Such information is necessary for the retrospective establishment of both the fact of overexposure itself and for an adequate assessment of the dose received. Translocations made up the bulk of the recorded colored rearrangements. Their frequency was significantly (3.3 times) higher than the level of unstable aberrations (dicentrics and acentrics). At the same time, there was a moderate correlation between the level of FISH translocations and the age of the donors, and gender did not affect this indicator in any way. Also, no significant effect of various types of medical diagnostic radiation, smoking and the disease of the new COVID-19 coronavirus infection on the studied index of chromosome damage was found. In general, the detected frequencies of translocations in genome-equivalent cells in their relationship with age were in fairly good agreement with the results of international studies.