Mechanistic Underlying Anti-diabetic Potential of Eclipta alba Flavonoids against Aldose Reductase Inhibitory Action

Background: One of the main factors contributing to morbidity and mortality in the world's population is diabetes mellitus. The primary symptoms of this condition are hyperglycemia, or high blood sugar, which is followed by polydipsia and polyuria. Damage to the retina, loss of kidney function, and nerve damage are examples of subsequent consequences. Moreover, diabetes mellitus will accelerate the development of cardiovascular disease. Eclipta alba (L.) Hassk. (Also known as Eclipta prostrata Roxb.) belongs to the Asteraceae family and is commonly known as false daisy in English and bhringoraj or bhringraj in Bangladesh and India. It is regarded as a weed of ethnomedicinal significance. Pharmacological activities of plant extracts and individual phytoconstituents have revealed anticancer, hepatoprotective, snake venom neutralizing, anti-inflammatory, and antimicrobial properties. Purpose: This study aimed to assess the antidiabetic activity of Eclipta alba leaf flavonoid through in-silico molecular docking. Method: Aldose reductase was chosen as the target proteins in the current investigation. The bond was found using the Auto Dock software using a grid-based docking method. Compounds' 2D structures were generated, converted to 3D, and subsequently energetically lowered up to an arms gradient of 0.01 using the Merck Molecular Force Field (MMFF). Result: Flavonoids of E.alba found to be effective anti-diabetic component and effectively binds to be target protein Aldose reductase with binding energy -8.96, -6.02 & -5.86 kcalmol-1 for apigenin, syringic acid & vanillic acid respectively. Conclusion: The finding of the in-silico molecular docking showed that both lead compound is effective binds & inhibitory action on target protein. The molecular docking of ligands like apigenin, syringic acid & vanillic acid with Aldose reductase receptor revealed that it has exhibited the chemical interaction with the amino acids in the active pockets.