A novel simplified structural design as an artificial enzyme for efficient hydrolysis of PNPA
In this study, a novel artificial enzyme Zn(II)-SMM with simple organic structure for PNPA-directed hydrolysis is proposed. The introduction of hydrophobic quinoline and dipyridinium in Zn(II)-SMM system demonstrates high activity for the complexation of Zn<sup>2+</sup> and hydrolysis of PNPA, and the catalytic rate (k<sub>cat</sub>/k<sub>uncat</sub>) exceeds 5239 times that of non-catalytic systems. The newly designed small molecule organic complex effectively binds to and catalyzes the hydrolysis of substrate PNPA in accordance with Michaelis-Menten kinetics. The mechanism with activation by single Lewis acid provides fundamental insights for the development of small molecule hydrolases.
Authors
- Chung‐Yi Li (ORCID: https://orcid.org/0000-0002-0321-8908)
- Jiajun Wang (ORCID: https://orcid.org/0000-0002-1269-0366)
- Wenfang Li (ORCID: https://orcid.org/0009-0002-5003-8157)
- Yao-dong Lu
Institutions
- Jilin Normal University (CN)
Publication Details
- Journal
- Scientific Reports
- Published
- 2025-03-17
- DOI
- https://doi.org/10.1038/s41598-025-92439-1
- Citations
- 3
- Primary Topic
- Advanced Nanomaterials in Catalysis
- Type
- article
- Field-Weighted Citation Impact
- 1.55
Funders
- National Natural Science Foundation of China