Continuous flow-through steady state system for in vitro characterization of CYP11B2 inhibitors–Impact on enzyme kinetics of steroidogenesis
Our flow-through system provides insights into inhibitor-induced alterations of metabolic fluxes and enzymatic rate constants, and thus represents an improved preclinical model sytem for complex characterization of CYP11B2 inhibitors.
Authors
- Peter Dieterich (ORCID: https://orcid.org/0000-0002-3564-0193)
- Mirko Peitzsch (ORCID: https://orcid.org/0000-0002-2472-675X)
- Tracy Ann Williams (ORCID: https://orcid.org/0000-0002-2388-6444)
- Sanas Mir-Bashiri (ORCID: https://orcid.org/0009-0008-9051-1424)
- Stefanie Hahner (ORCID: https://orcid.org/0000-0003-1616-8986)
- Graeme Eisenhofer (ORCID: https://orcid.org/0000-0002-8601-9903)
- Dennis Fröbel (ORCID: https://orcid.org/0009-0002-2463-0819)
- Andreas Schirbel (ORCID: https://orcid.org/0000-0002-3085-7561)
- Nicole Bechmann (ORCID: https://orcid.org/0000-0002-6932-333X)
- Georgiana Constantinescu (ORCID: https://orcid.org/0000-0002-7304-2557)
- Britta Heinze (ORCID: https://orcid.org/0009-0001-2060-4851)
Institutions
- LMU Klinikum (DE)
- Universitätsklinikum Würzburg (DE)
- University Hospital Carl Gustav Carus (DE)
- Technische Universität Dresden (DE)
- Ludwig-Maximilians-Universität München (DE)
Publication Details
- Journal
- The Journal of Steroid Biochemistry and Molecular Biology
- Published
- 2025-03-16
- DOI
- https://doi.org/10.1016/j.jsbmb.2025.106736
- Citations
- 1
- Primary Topic
- Hormonal Regulation and Hypertension
- Type
- article
- Field-Weighted Citation Impact
- 1.29