Protocol for capturing genome-wide chromatin interactions in Arabidopsis
High-order chromatin structure is essential for the regulation of plant growth and development. Here, we present a protocol for capturing genome-wide chromatin interactions in Arabidopsis. We describe steps for tissue fixation, nuclei isolation, chromatin digestion, ligation, sonication, and library preparation. This protocol has potential application in 3D chromatin analysis in many species of plants. For complete details on the use and execution of this protocol, please refer to Shu et al.,<sup>1</sup> Yin et al.,<sup>2</sup> and Yang et al.<sup>3</sup><sup>,</sup><sup>4</sup>.
Authors
- Shi Xiao (ORCID: https://orcid.org/0000-0002-6632-8952)
- Dingyue Wang (ORCID: https://orcid.org/0000-0002-1027-7455)
- Yue Zhou (ORCID: https://orcid.org/0000-0002-2507-7990)
- Jiayue Shu (ORCID: https://orcid.org/0000-0003-2939-0076)
- Minqi Yang (ORCID: https://orcid.org/0000-0003-3467-3759)
Institutions
- Peking University (CN)
- Center for Life Sciences (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-03-16
- DOI
- https://doi.org/10.1016/j.xpro.2025.103702
- Citations
- 1
- Primary Topic
- Chromosomal and Genetic Variations
- Type
- article
- Field-Weighted Citation Impact
- 1.75