IMPACT OF ATMOSPHERIC PH AND INCUBATION TIME ON INVITRO BIOFILMS OF LIMOSILACTOBACILLUS REUTERI AND LACTICASEIBACILLUS RHAMNOSUS

Participation of the microbiome in the evolution of chronic infectious and non-infectious processes has been described by numerous authors.One of the ways to modulate it is through the use of probiotics (PB).It is important that these PB have the ability to form biofilm (BP) in different mucous membranes of the body.The microorganisms most frequently used as probiotics are Lactobacillus spp and related microorganisms.Lactobacilli are strictly aerotolerant or anaerobic grampositive bacilli that are part of the human gastrointestinal and genitourinary microbiota.There are studies that show that probiotic properties are closely linked to the species and strain 1 .There are many probiotics with proven efficiency in the gastrointestinal tract but the same does not occur with probiotics at the urogenital level.In addition to the characteristics that are always listed such as the production of hydrogen peroxide, lactic acid, hemolytic activity, presence of resistance genes capable of being transferred It is necessary to establish characteristics in relation to the habitat such as tolerance to different pH, presence of oxygen and capacity to form biofilms (BP) that ensure their persistence over time.It must be taken into account that these parameters are very different at the intestinal level vs. the vaginal level.Therefore, it cannot be assumed a priori that the probiotics used to modulate the microbiota of a mucosa are the same to do so in another different mucosa.