The effect of abamectin exposure on gametogenesis in zebrafish

Today, pesticides are widely used to enhance agricultural yield mixed with soil and water, creating significant environmental pollution. The extensive use of insecticides for pest control has made this issue more pronounced. Abamectin, a key member of the avermectin family, is used as an insecticide and an antihelminthic agent in agriculture. It is an active and effective agricultural pesticide, particularly preferred for combating pests such as aphids and red spider mites. The dosage and frequency of its use vary depending on the target plant species and pest organism. For example, abamectin-based products with an 18 g/L EC formulation in apple orchards are typically recommended at 10 ml/100 L of water to control red spider mites. Although significantly below agricultural application levels, the low dose of 0.75 µg L⁻¹ used in our study has demonstrated effects that cannot be overlooked. However, it can contaminate aquatic environments, posing harmful effects on organisms. Studies indicate that abamectin exposure may lead to serious health issues, showing toxic and reproductive toxicity effects in aquatic species. Examining abamectin's effects on testicular tissue revealed hypertrophy of Sertoli cells in the group exposed to 0.75 µg L⁻¹ of abamectin. Apoptotic cells were observed in the groups exposed to 0.75 µg L⁻¹ and 1.5 µg L⁻¹. At the same time, pyknotic structures, disruption of seminiferous tubules, interstitial fibrosis, and atrophic appearance were identified across all dose groups, with severity increasing dose-dependently. Analysis of ovarian tissue demonstrated distortion of the zona radiata in groups exposed to 0.75 µg L⁻¹ and 1.5 µg L⁻¹ of abamectin. Moreover, in all dose groups, thickening of the zona radiata, vacuolization, formation of degenerated follicles, and nuclear disruption were observed, with these pathological alterations exacerbating in a dose-dependent manner. Like many studies involving zebrafish, this research is crucial for assessing potential toxic effects that may pose risks to human health. This study examined the histopathological effects of varying doses of abamectin (0.75 µg L⁻¹, 1.5 µg L⁻¹, and 3 µg L⁻¹) on zebrafish gonads after 96 h of exposure. Using standard histological techniques, the samples prepared were stained with H&E and observed under a light microscope. Statistical analyses were conducted using SPSS 23. The normality of the data was assessed with the Shapiro-Wilk test. One-way ANOVA and Tukey post-hoc tests were used for normally distributed groups, while the Kruskal-Wallis and Dunnett's T3 tests were applied for non-normally distributed groups. All analyses were performed with a 95% confidence interval and a significance level of p > 0.05.