Acetol biosynthesis enables NADPH balance during nitrogen limitation in engineered Escherichia coli
The results reported in this study illustrate how the production of a value-added chemical from a waste stream can be connected to the metabolism of the whole-cell biocatalyst, making product formation mandatory for the cell to maintain its NADPH/NADP<sup>+</sup> balance. This has implications for process design and further metabolic engineering of the whole-cell biocatalyst.
Authors
- Philip Mennicken
- Philipp Demling (ORCID: https://orcid.org/0000-0002-0351-1119)
- Lars M. Blank (ORCID: https://orcid.org/0000-0003-0961-4976)
- Birgitta E. Ebert (ORCID: https://orcid.org/0000-0001-9425-7509)
- Suresh Sudarsan (ORCID: https://orcid.org/0000-0002-2985-1728)
- Joerg M. Buescher (ORCID: https://orcid.org/0000-0002-6547-0076)
- Emre Özdemir (ORCID: https://orcid.org/0000-0001-5548-6720)
- Achraf Ammar (ORCID: https://orcid.org/0000-0003-0347-8053)
- Guido Meurer
Institutions
- The University of Queensland (AU)
- Novo Nordisk Foundation (DK)
- Brain (Germany) (DE)
- Max Planck Institute of Immunobiology and Epigenetics (DE)
- RWTH Aachen University (DE)
- Technical University of Denmark (DK)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-03-16
- DOI
- https://doi.org/10.1186/s12934-025-02687-z
- Citations
- 3
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 2.42
Funders
- Bundesministerium für Bildung und Forschung
- RWTH Aachen University