Investigations on the Stimulating Effect of the Combination extracts of Moringa oleifera and Piper nigrum L. on Isolated Rat Leydig Cells for Testosterone Biogenesis

Moringa oleifera (MO) and Piper nigrum L. (PN), Vajikarana drugs of Ayurveda, are listed to cure male infertility. The objective of this study was to investigate the stimulating effect of Moringa oleifera extract in combination with the different doses of PN extract on isolated Rat Leydig cells for testosterone biogenesis. Isolated Leydig cells were incubated with various doses of MO extract (10, 100, and 1000 μg/ml) as well as a combination with PN extract at doses of 5, 10, and 20%. The testosterone level will be measured by reported HPTLC method. Results indicated that 100 μg/ml of MO extract was effective as compared to the other two doses, but in combination with PN extract at 5, 10 and 20 % levels, the testosterone concentration increased at 10, and 100 μg/ml concentration in contrast to 1000 μg/ml concentration significantly compared to the cells treated with MO extract alone at similar doses. Overall, results of the study indicated that PN in combination of MO could be able to stimulate Leydig cells for testosterone biogenesis.