Effect of commercial herbal-extract products Tetrayou® and Feed Ad® on the survival of white shrimp, Penaeus vannamei challenged with pathogenic Vibrio parahaemolyticus-AHPND strain

Acute hepatopancreatic necrosis disease (AHPND) is the most critical shrimp disease due to the economic losses registered worldwide. Herbal remedy is one of the most important eco-friendly strategies due to its content of natural bioactive compounds than can act against bacterial diseases. In the present study, organisms of P. vannamei were challenged with a strain of V. parahaemolyticus-AHPND. A simple experimental design was performed to compare the survival rate among five different commercial herbal extracts concentrations of Tetrayou® and Feed Ad® against a control treatment. Gross observation and histopathology were carried out on moribund organisms. Significant differences were observed (p<0.05) where organisms fed with T5 (400 mg Tetrayou®/Kg of commercial feed + 1g Feed Ad®/Kg of commercial feed), showed the highest survival value (73 ± 5.8%) in comparison to the rest of experimental treatments. Gross observation showed the characteristic signs of AHPND which were supported by histopathological analysis. It was concluded that Tetrayou® and Feed Ad® are suitable products to enhance the survival rate of P. vannamei when exposed to AHPND.