Inhibition Effect of Some Plant Extracts on Calliteara pudibunda (Linneaus, 1758) Acetylcholinesterase

In pest control management, acetylcholinesterase inhibition is one of the important methods. This study determined the optimum conditions of acetylcholinesterase in C. pudibunda and investigated some of its kinetic properties. Acetylcholinesterase inhibition studies were carried out with known inhibitors of acetylcholinesterase such as tacrine, edrophonium chloride, cypermethrin and aqueous extracts of olive leaf, walnut leaf, walnut shell, cherry laurel leaf and alder leaves. The Vmax and Km values of acetylcholinesterase, which showed maximum activity at 40.0 oC and pH 7.0, were determined as 1.7±0.2 EU and 0.18±0.02 mM, respectively. In inhibition studies, the IC50 values of tacrine, edrophonium chloride and cypermethrin were found to be 6.5±0.2, 2.8± 0.3 and 6.0±0.8 μM, respectively. The IC50 values of aqueous extracts of olive leaf, alder leaf, cherry laurel leaf, walnut shell and walnut leaf were found to be 1.8± 0.2, 1.8±0.4, 1.9±0.4, 2.8±0.6 and 5.8±1.2 µg dry matter/mL, respectively. In addition, these plant extracts' oleuropein and phenolic substance amounts were determined and correlated with IC50 values. As a result, these plant extracts used in the study can be recommended as an alternative biopesticide source to control such pests through acetylcholinesterase inhibition.