Study of Genetic Relatedness and Diversity of Vigna L. Accessories using ISRR and RAPD Markers

Molecular genetic studies were performed on 30 samples belonging to 2 species of the genus Vigna - V. unguiculata and V. radiata. However, since clear lines were not obtained for 2 samples representing the species V. radiata, the analyses were continued on 28 genotypes of V. unguiculata. Initially, the polymerase chain reaction was carried out with 5 ISSR primers, with 2 of which (UBC 810 and UBC 812) no amplification product was synthesized for more than half of the samples, whereas for the remaining 3 samples amplicons were obtained for most samples. Using primers UBC 810 and UBC 812, 8 (5 polymorphs) and 6 (3 polymorphs) sequences of 250–900 bp in length were synthesized, respectively. Another profile was obtained for sample #12 AG-342 with marker UBC 810 with the sequence (GA)8T.