Artificial base mismatches-mediated PCR (ABM-PCR) for detecting clinically relevant single-base mutations
This study highlights the impact of primer-template mismatches on PCR amplification and provides insights into rational design of effective ABM-PCR primers for detecting single-base mutations with high specificity and sensitivity. It is highly valuable for clinical diagnosis and prognosis use.
Authors
- Jimmy Xiangji Huang (ORCID: https://orcid.org/0000-0003-1292-1491)
- Pik Kwan Lo (ORCID: https://orcid.org/0000-0001-5255-1718)
- Haibao Zhu (ORCID: https://orcid.org/0009-0005-1982-678X)
- Chung Tin (ORCID: https://orcid.org/0000-0001-5337-2578)
- Jiaqi Li (ORCID: https://orcid.org/0009-0005-5564-6241)
- Zhihao Huang (ORCID: https://orcid.org/0000-0003-2638-8237)
- Ming Xu (ORCID: https://orcid.org/0000-0002-5215-8892)
- Zhongqi Zhou
- Gang Chen (ORCID: https://orcid.org/0000-0002-4864-1451)
- Tao Wang (ORCID: https://orcid.org/0000-0003-0465-3167)
- Hongman Xue
- Yumei Huang (ORCID: https://orcid.org/0009-0009-6376-5362)
- Cia‐Hin Lau (ORCID: https://orcid.org/0000-0002-4528-0363)
- Wenjiao Dong
- Kangxian Guo
- Minghai Zou
Institutions
- Sun Yat-sen University (CN)
- City University of Hong Kong (HK)
- Guangdong Medical College (CN)
- Shantou University (CN)
- The Seventh Affiliated Hospital of Sun Yat-sen University (CN)
- Fujian Provincial Cancer Hospital (CN)
Publication Details
- Journal
- Clinical Chemistry and Laboratory Medicine (CCLM)
- Published
- 2025-03-15
- DOI
- https://doi.org/10.1515/cclm-2024-0962
- Citations
- 2
- Primary Topic
- Cancer Genomics and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 1.87
Funders
- City University of Hong Kong
- Science, Technology and Innovation Commission of Shenzhen Municipality
- Sanming Project of Medicine in Shenzhen