IN-SILICO ANALYSIS OF PATHOGENESIS-RELATED PROTEINS (PR-1) IN BARLEY (HORDEUM VULGARE L.)

A voide range of proteins is induced in the plant, once the defensed is alarmed including pathogenesis related proteins (PRs).The aim of my research was to study understand the role and possible function of PRs in plant defense.The studies are mainly focused on PR-1 CAP domain.The CAP domain was confirmed as being highly conserved in all these PR-1s.These PRs accumulated in both leaves and roots of barley seedlings as a response to infection of the respective tissues.Phylogeny-based inference exposed that PR-1 proteins clustered into five major groups, with the majority of PR-1s distributed in Group A (1 out 15), Group B and C (2 PR-1 each out of 15), Group D (4 out 15) and the Group E (6 out 15) respectively.PRs defense system of plants may respond and could be categorized into various families according to similarities in their sequences, enzyme-related properties and structural aspects.PRs appear to be part of a preformed defense since PR-1 was constitutively present in both xylem and phloem tissues and the root epidermis.These results suggest that different induction strategies may be active in Hordeum vulgare seedlings depending on the primary site of infection by B. sorokiniana.