Metagenomic detection of protozoan parasites on leafy greens aided by a rapid and efficient DNA extraction protocol
Our metagenomic procedure led to the identification of <i>C. parvum</i> present on lettuce at low numbers and successfully identified and differentiated other protozoa either of the same genus or of different genera. This novel mNGS assay has the potential for application as a single universal test for the detection of foodborne parasites, and the subtyping of parasites for foodborne outbreak investigations and surveillance studies.
Authors
- Sarah J. Reiling (ORCID: https://orcid.org/0000-0003-4444-0457)
- Sohail Naushad (ORCID: https://orcid.org/0000-0001-8219-1717)
- Andrée Ann Dupras
- Brent R. Dixon (ORCID: https://orcid.org/0000-0002-0390-9661)
- Dele Ogunremi (ORCID: https://orcid.org/0000-0001-9123-5574)
- Marc‐Olivier Duceppe (ORCID: https://orcid.org/0000-0003-2130-0427)
- Ruimin Gao (ORCID: https://orcid.org/0000-0002-4949-1897)
- Harriet Merks
Institutions
- Health Canada (CA)
- Canadian Food Inspection Agency (CA)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2025-03-14
- DOI
- https://doi.org/10.3389/fmicb.2025.1566579
- Citations
- 3
- Primary Topic
- Parasitic Infections and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 6.14