Effect of <i>Palisada perforate</i> (Bory) K.W.Nam supplementation on gas production and fermentation kinetics in total mixed ration

This research aimed to evaluate Palisada perforate (Bory) K.W.Nam as a supplement on total mixed ration to their gas production and fermentation kinetics. A factorial randomized block design with two factors and five replications. The first factor was kinds of total mixed ration (TMR 1, 80% grass (G) and 20% concentrate (C) based on dry matter (DM); TRM 2, 70% G and 30% C; TMR 3, 60% G and 40% C) and the second factor was level of P. perforate (Bory) K.W.Nam supplementation (0 and 5% organic matter (OM)). There was no interaction between kinds of TMR and level of P. perforate (Bory) K.W.Nam supplementation on gas production and fermentation kinetics. The TMR 3 increased (P&lt;0.01) gas production in early incubation (2 and 12 h) but was similar to all treatments on 24 and 48 h of incubation. Meanwhile, increased concentrate proportion on TMR 2 and 3 increased (P&lt;0.01) easily degraded fraction (a). Supplementation of P. perforate (Bory) K.W.Nam up to 5% OM on all TMR doesn’t affect gas production and fermentation kinetics. TMR consisted of 60% grass and 40% concentrate is the best basal diet to increase gas production in early incubation and easily degraded fraction on fermentation kinetics.