A Simple Approach to Assign Disulfide Connectivity for Ziconotide via Partial Reduction Without Alkylation
A relatively simple method based on partial reduction without alkylation, followed by analysis with liquid chromatography-tandem mass spectrometry, was developed, assigning disulfide connectivity for a disulfide-rich peptide. This method is useful in the quality control of medicinal peptides with an extensive disulfide network.
Authors
- Peize Wu
- Ming Li (ORCID: https://orcid.org/0000-0002-6762-2491)
- Weihua Wang (ORCID: https://orcid.org/0000-0002-6503-2164)
- Yuya Cheng (ORCID: https://orcid.org/0009-0007-3213-6825)
Institutions
- National Institute of Metrology (CN)
Publication Details
- Journal
- Rapid Communications in Mass Spectrometry
- Published
- 2025-03-14
- DOI
- https://doi.org/10.1002/rcm.10026
- Citations
- 1
- Primary Topic
- Chemical Synthesis and Analysis
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Key Research and Development Program of China