Gastro-protective Effect of Ageratum conyzoides (Linn) Leaf Extract on Aspirin-Induced Ulcer in Albino Rats

his study examined the protective effects of Ageratum conyzoides leaf extract on ulcers in albino rats. Thirty-two male rats were divided into two groups: twelve for the acute toxicity study and twenty for the ulcer experiment, divided into four groups. Group A (normal control) received 2ml of distilled water and 0.03ml Tween 80. Group B (negative control) was administered 400mg/kg aspirin. Group C was treated with 20mg/kg omeprazole and 400mg/kg aspirin, while Group D received 500mg/kg of Ageratum conyzoides extract and 400mg/kg aspirin. Rats were fasted for 24 hours with access to water, and Groups C and D received their respective treatments 30 minutes before aspirin. No mortality was recorded in the acute toxicity test, even at 5000mg/kg. Gastric juice volume significantly increased in Group B but decreased in Groups C and D (p<0.05). pH levels dropped significantly in Group B (p<0.05), while free acidity, total acidity, and pepsin activity increased, indicating ulceration. These values were reduced in Groups C and D. Ulcer index increased in Group B, but the percentage inhibition in Groups C and D were 62% and 66%, respectively, compared to Group B. The anti-ulcerogenic effects of Ageratum conyzoides could be linked to its bioactive compounds. The novelty of the study is that Ageratum conyzoides (500 mg/kg) provides better gastro-protection (66% inhibition) than omeprazole (62%) in aspirin-induced ulceration. It also emerges as a potent, natural, and cost-effective alternative to synthetic anti-ulcer drugs. This study supports its further development for ulcer management.