High-Quality Application of Crayfish Muscle in Surimi Gels: Fortification of Blended Gels by Transglutaminase

The application of crayfish muscle in surimi products is a potential way to promote their processing and ensure that it is of a high value. In this study, a one-way completely randomized design was used to prepare mixed surimi gels with different proportions of crayfish muscle. The effect of transglutaminase (TGase) on the improvement in the structural properties, water-binding capacity, micromorphology and protein conformation of blended gels was explored using mass spectrometry, centrifugation, scanning electron microscopy, and Fourier transform infrared spectroscopy. The results of thus study were analyzed by one-way ANOVA showed that in the absence of TGase, crayfish muscle made the microstructure of the blended gel looser and rougher, with a reduction in the strength of the gel and a decrease in the water holding capacity. The addition of 0.6% TGase was able to ameliorate this negative effect by promoting the formation of key chemical bonds and changes in protein conformation, which ultimately led to the enhancement of the crayfish-surimi blended gel properties. Practically, this study provides a viable strategy for incorporating crayfish into surimi products, enabling the development of novel, high-quality seafood products with improved texture and moisture retention, thereby enhancing consumer appeal and reducing waste in crayfish processing.