The kinase CPK5 phosphorylates MICRORCHIDIA1 to promote broad-spectrum disease resistance

In Arabidopsis (Arabidopsis thaliana), MICRORCHIDIA 1 (MORC1), a member of the MORC family of evolutionarily conserved GHKL-type ATPases, plays important roles in multiple layers of plant immunity. However, the molecular mechanism by which MORC1 regulates plant immunity remains obscure. Here, we report that the pathogen-responsive kinase CALCIUM-DEPENDENT PROTEIN KINASE 5 (CPK5) directly interacts with and phosphorylates MORC1, thereby promoting its stability and nuclear translocation. In the nucleus, MORC1 associates with the NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1)-TGACG-BINDING FACTOR (TGA) transcriptional complex to upregulate defense-responsive genes and promote plant resistance against several pathogens, such as the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 and fungal pathogen Botrytis cinerea. Therefore, this study uncovers a MORC1-mediated immune signaling pathway, in which the CPK5-MORC1-NPR1-TGA module transduces Ca2+ signals, leading to the upregulation of defense genes involved in plant immunity.

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Publication Details

Journal
The Plant Cell
Published
2025-03-01
DOI
https://doi.org/10.1093/plcell/koaf051
Citations
8
Primary Topic
Plant-Microbe Interactions and Immunity
Type
article
Field-Weighted Citation Impact
14.00

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article

The kinase CPK5 phosphorylates MICRORCHIDIA1 to promote broad-spectrum disease resistance

Zhipeng Tu, Yongming Chen, Guangyuan Xu, Guozhi Bi et al.
8 citations
The Plant Cell
Plant-Microbe Interactions and Immunity
14.00
article

The kinase CPK5 phosphorylates MICRORCHIDIA1 to promote broad-spectrum disease resistance

Zhipeng Tu, Yongming Chen, Guangyuan Xu, Guozhi Bi, Xiangzong Meng, Jun Fan, Xiaodan Wang, Xiangxiu Liang, Aifang Ma, Daolong Dou, Congcong Sun, Tingting Zhao, Yingying Song, Pan Wang, Jing Pan
article en
8 citations

Abstract

In Arabidopsis (Arabidopsis thaliana), MICRORCHIDIA 1 (MORC1), a member of the MORC family of evolutionarily conserved GHKL-type ATPases, plays important roles in multiple layers of plant immunity. However, the molecular mechanism by which MORC1 regulates plant immunity remains obscure. Here, we report that the pathogen-responsive kinase CALCIUM-DEPENDENT PROTEIN KINASE 5 (CPK5) directly interacts with and phosphorylates MORC1, thereby promoting its stability and nuclear translocation. In the nucleus, MORC1 associates with the NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1)-TGACG-BINDING FACTOR (TGA) transcriptional complex to upregulate defense-responsive genes and promote plant resistance against several pathogens, such as the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 and fungal pathogen Botrytis cinerea. Therefore, this study uncovers a MORC1-mediated immune signaling pathway, in which the CPK5-MORC1-NPR1-TGA module transduces Ca2+ signals, leading to the upregulation of defense genes involved in plant immunity.

The Plant CellVol. 37(3)
South China Agricultural University (CN), Nanjing Agricultural University (CN), Shanghai Normal University (CN), China Agricultural University (CN)
Shandong University, National Natural Science Foundation of China, China Agricultural University
Openalex Percentile: Top 1%
Plant-Microbe Interactions and Immunity
14.00
DOIOpen Access PDFOpenAlex
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