Protocol for mouse carotid artery perfusion for in situ brain tissue fixation and parallel unfixed tissue collection
As the study of central control of multiple organ function becomes more prominent, there is an increasing need for the collection of fixed brain and unfixed organs and tissues from the same experimental animal. Here, we present a protocol for performing carotid artery cannulation, organ and tissue collection, in situ brain perfusion and fixation, and brain dissection in mice. We describe steps for cannulating the carotid artery, harvesting the heart and other organs, and perfusing, fixing, and dissecting the brain.
Authors
- Chaoyin Chen (ORCID: https://orcid.org/0000-0001-6114-230X)
- Nipavan Chiamvimonvat (ORCID: https://orcid.org/0000-0001-9499-8817)
- Phung Ngoc Thai (ORCID: https://orcid.org/0000-0001-7344-150X)
- Valeriy Timofeev
- Zubayer Ibne Ferdous (ORCID: https://orcid.org/0000-0003-1464-677X)
- Daphne A. Diloretto (ORCID: https://orcid.org/0000-0002-2386-2119)
- Xiao-Dong Zhang (ORCID: https://orcid.org/0000-0002-1042-8823)
- Ning Zong
Institutions
- University of Phoenix (US)
- University of California, Davis (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-03-14
- DOI
- https://doi.org/10.1016/j.xpro.2025.103699
- Primary Topic
- Neuroscience of respiration and sleep
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Veterans Affairs
- American Heart Association
- National Institutes of Health