Explorative 3-D culture of early secondary follicles in a time lapse system for up to 36 days gives valuable, but limited insights in follicular development

Time lapse monitoring of 3-D cultured follicles is a promising explorative approach to obtain valuable visual insights regarding the many facets of follicular growth and to optimize follicular culture conditions towards a clinical application. As the study is limited by a lack of mechanistic insights into theca cell differentiation and filopodia function, additional studies are necessary to validate the preliminary results of this approach.