Study on Extraction Technology of Pueraria Flavone and Its Hepatoprotective Effect

Gegen Root Yellow Copper, as a traditional Chinese medicine, contains various active ingredients, such as flavonoids, ginsenosides, etc. It has antioxidant, anti-inflammatory, and liver-protective biological activities. With the increasing intake of toxins, drugs, and alcohol in modern life, the liver's burden has become heavier, and the incidence of liver diseases has gradually increased. Therefore, it has become particularly important to find natural drugs with liver-protective effects. The total flavonoids of Pueraria Montana(Loureiro)Merrill) were extracted by a single factor test combined with response surface method. The total flavonoids of Pueraria were characterized by UV spectrophotometry and high performance liquid chromatography. AGAR diffusion method and DPPH, ABTS+- and total reduction capacity were used to evaluate the physical activity of total flavonoids from Pueraria. The results showed that the optimized extraction process of total flavonoids from Pueraria root was as follows: solid-liquid ratio (m:V.g:mL)1:16, extraction time was 69min, extraction time was 3 times, and the yield of total flavonoids was 7.690%. The total flavonoids of Pueraria root included 9 kinds of common flavonoids, accounting for 91.81% of the total content. Animal experiments showed that Pueraria root flavonoids could significantly reduce the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and the content of propyl (MDA) in serum of mice. Increased the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in the liver tissue of mice, and had an obvious hepatoprotective effect. The results can provide a reference for the extraction and application of Gerona.