Expression and localization of HBsAg in corneas from HBsAg<b>-</b>seropositive donors preserved in medium<b>-</b>term preservation solution and glycerol.

HBsAg-positive expression persists in the epithelium or endothelium of donor corneas from HBsAg-seropositive individuals, regardless of preservation in medium-term preservation solution or glycerol. However, no HBsAg expression was detected in the corneal stroma. Higher blood HBV DNA titers correlate with increased likelihood of HBsAg positivity in corneal tissues. This study lays the groundwork for further investigation into the potential use of HBsAg-positive donor corneas in lamellar keratoplasty.