ANALYSIS OF TRANSCRIPTIONAL ACTIVITY OF HUMAN MUCIN5AC GENE UNDER THE INFLUENCE OF V.CHOLERAEGBPA PROTEIN

GlcNAc binding protein A (GbpA) is a secretory protein of Vibrio.cholerae that facilitates its adherence to human intestine. Binding of V.cholerae to intestinal mucin by GbpA results in increased mucus secretion which attracts more bacteria for better colonization. However, the molecular insights of GbpA induced mucin production in host cells is yet to be investigated. Based on this knowledge gap the objective of this study was set to elucidate the role of GbpAin inducing Mucin5AC gene in HT-29 cell line and to identify the transcription factor binding sites of Mucin5AC gene involved in the activation process. To study the transcriptional activity of the Mucin5AC gene under the influence of GbpA, Luciferase-reporter gene assay was performed at varying conditions. The upregulation of Mucin5AC promoter activity was found dose-dependent and interestingly the upstream region between -324 bp to -64 bp of Mucin5AC was prerequisite for the GbpA-induced trans-activation. Thus, this study provide an insight into the GbpA induced mucin secretion at a molecular level which ultimately reinforce the concept of successful intestinal colonization and pathogenesis by V.cholerae that could lead to therapeutic approaches for blocking bacterially-induced mucin overproduction.