Development of eugenol-fortified fisetin-loaded nano-invasomes gel

The goal of current investigation was to develop eugenol-fortified fisetin nano-invasomes. Fisetin-loaded invasomes were prepared using thin film hydration procedure and evaluated for various parameters. Additionally, the optimised fisetin invasomes formulation (F5) was converted to fisetin invasomes gel using Carbopol^® as gelling agent and evaluated for pH, spreadability, homogeneity, drug content, <i>in vitro</i> fisetin release, antioxidant activity and stability study.Prepared optimised fisetin invasomes formulation (F5) demonstrated vesicles size, PDI, zeta potential and entrapment efficiency of 153.85 ± 14.32 nm, 0.208 ± 0.042, -12.67 ± 1.08 mV and 72.10 ± 6.36%. The TEM image indicated that the prepared invasomes vesicles are intact, spherical and found in the range of nanosized scale. Prepared fisetin invasomes gel showed better spreadability and <i>in vitro</i> fisetin released in contrast to fisetin control gel. Substantial improvement in the DPPH radical scavenging activity of fisetin invasomes gel 44.70% (3.1 µM) and 83.94% (50 µM), was noted as compared to the control gel at 39.47% (3.1 µM) and 79.10% at (50 µM). The prepared fisetin invasomes gel formulation was found stable at 4 °C.Based on the results, prepared invasomes gel formulation was found as a viable method for better delivery of bioactive compound(s) including fisetin.