Alpha-Amylase Inhibitory Activity of <i>Clerodendrum infortunatum</i> Plant as Natural Source for Diabetes Management

Owing to sedentary lifestyle, the increasing diabetes people in human society is a major concern for all the country. There are several synthetic anti-diabetic medicine and insulin are available in market for diabetes disease. However, the development of insulin resistance and side effects of synthetic medicine are alarming to look for natural plant-based medicine. The present study aimed to assess active compound present in Clerodendrum infortunatum leaf extract a novel agent for inhibition of alpha-amylase for the treatment of diabetes. Ethyl acetate and acetone solvent were used for the extraction of secondary metabolite from dried C. infortunatum leaf. Different concentrated of plant extracts were added in mixture of 10mM phosphate buffer (pH 7), starch (2mg/ml) and 50 U alpha-amylase enzyme for inhibition study. Ethyl acetate and acetone extract are inhibited the alpha-amylase by 79.1±0.9 and 40±1.8% respectively at 4mg/ml concentration. Ethyl acetate extract was loaded on TLC and four distinct band B1, B2, B3 and B4 band are purified for alpha-amylase inhibition study. The maximum inhibition was observed in band B4 (41%) followed by B1, B2 and B3. TLC purified compound was belonging to phenolic group of compounds in LC-MS analysis. The present study revealed the alpha-amylase inhibition activity by ethyl acetate extract of C. infortunatum leaf and TLC purified compound. This extract can be considered as an alternative to current available medicine or development of herbal based formulation.