Green Synthesis of Silver Nanoparticle using Ethanolic Extract of <i>Boerhavia diffusa</i> and it’s <i>in vitro</i> Antioxidant Activity

The prospective uses of biological entities in the green production of silver nanoparticles (AgNPs) in nanomedicine are attracting significant interests. The current study focuses on synthesizing AgNPs using the ethanolic extract of Boerhavia diffusa (B.diffusa) (EAgNPsBd), characterized by SEM-EDAX and Zeta-potential and the antioxidant activity of EAgNPsBd was assessed using DPPH, ABTS and FRAP assays. SEM-EDAX analysis confirmed the spherical and nano-sized nature of the synthesized EAgNPsBd, while Zeta-potential indicated a single peak of (-20.2 mV). The DPPH and ABTS assays, EAgNPsBd exhibited significantly lower IC50 values (20.8 μg/mL and 62.1 μg/mL, respectively) compared to standard ascorbic acid (65.6 μg/mL) and quercetin (84.8 μg/mL), as observed in the FRAP assay. Significantly higher antioxidant activity is implied by lower IC50 values. The in vitro antioxidant activities demonstrated the excellent radical scavenging capabilities of EAgNPsBd. This study emphasises the safe and environmentally responsible use of EAgNPsBd in prospective therapeutic, nanobiotechnological and biomedical applications, especially in context to diabetes mellitus.